Our research at Matriscience meets the needs of the pharmaceutical and dermo-cosmetic industry
Matriscience has developed a screening platform for active principles in 2010, followed by an analysis platform combining photobiomodulation and bioinduction in 2014, which was designed to reproduce the main steps of skin repair and healing based on in vitro models. These platforms have given rise to several active principles and patentable and/or patented processes.
Our research is based on conditioned-medium transfer technology
In order to generate a response from keratinocytes and to understand how they interact with fibroblasts, our team at Matriscience carries out in vitro trials based on primary cultures of human cells.
Various methods of induction used on skin cells, keratinocytes and fibroblasts make it possible to explore tissular bioinduction:
- photobiomodulation, in particular via exposure to stimulatory light (LED)
- bioinduction, or biological stimulation,
- mechanotransduction, which consists in converting a mechanical stimulus into an electrical signal
The trial can be broken down into 4 steps:
- photobiomodulation, in particular via exposure to stimulatory light (LED)
- bioinduction, or biological stimulation,
- mechanotransduction, which consists in converting a mechanical stimulus into an electrical signal
The trial can be broken down into 4 steps:
1) The keratinocytes are cultured then stimulated through one of the methods mentioned above.
2) The cell culture supernatant is harvested after 24 hours. Within it are present all the proteins produced by the keratinocytes. This is called the secretome.
3) The supernatant is then added to cultured fibroblasts.
4) The response, migration or proliferation of target cells is monitored 48 hours later.
2) The cell culture supernatant is harvested after 24 hours. Within it are present all the proteins produced by the keratinocytes. This is called the secretome.
3) The supernatant is then added to cultured fibroblasts.
4) The response, migration or proliferation of target cells is monitored 48 hours later.
The aim is to identify and confirm the effectiveness of new active principles
that are able to interact with these cell populations, thus inducing a measurable effect.
A photobiomodulation platform (LED): understanding the mechanism of action of light on the cell and approving innovative practical applications
Over the past few years, the emerging use of LEDs in low-level laser therapy has been a fast-growing field of study in the area of medical dermo-cosmetics, dermatology and scar surgery.
When a light wave stimulates a cell, it triggers a number of chemical and physical changes which influence cell activity: this is known as photobiomodulation. Photobiomodulation can be defined as the process whereby chromophores present in the cell selectively absorb the low-energy wavelengths and generate cell-signaling phenomena which are at the root of major biological syntheses and cell metabolism modifications.
When a light wave stimulates a cell, it triggers a number of chemical and physical changes which influence cell activity: this is known as photobiomodulation. Photobiomodulation can be defined as the process whereby chromophores present in the cell selectively absorb the low-energy wavelengths and generate cell-signaling phenomena which are at the root of major biological syntheses and cell metabolism modifications.
LED light therapy as explored in vitro through the Matriscience platform allows us to maximize illumination parameters such as wavelength, dosage (fluence), intensity (power density), duration of illumination (processing time), and the pulse or continuous mode of the generating electric current.
These parameters have an effect on tissular repair processes: scars, stretch marks, burns, acne treatment, chronic dermatoses…
Through the platform, we have developed innovative experimental devices that enable us to combine the effects of LEDs of varying wavelengths with active principles whose ability to induce a synthesis by dermal cells of, for example, collagen needs to be assessed.
These parameters have an effect on tissular repair processes: scars, stretch marks, burns, acne treatment, chronic dermatoses…
Through the platform, we have developed innovative experimental devices that enable us to combine the effects of LEDs of varying wavelengths with active principles whose ability to induce a synthesis by dermal cells of, for example, collagen needs to be assessed.
